Litmus Milk Procedural Essay Example for Free

Litmus Milk Procedural EssayLitmus draw is a complex medium that can potentially distinguish many species of bacteria. Litmus milk has several components that can be metabolized lactose (milk sugar) casein (milk protein) and litmus test (a pH indicator). If lactose is fermented, the solution should turn pink. If gas is produced during fermentation, you whitethorn be able to see bubbles or cracks in the milky medium. If lactose is not fermented and proteins argon metabolized instead, the solution will become alkaline and turn blue in color. If casein is digested, the milk will coagulate to form a curd (a solid). Casein may be metabolized all the way down to individual amino acids. This process, called peptonization, results in a clear (not milky) liquid that is usually brown in color. Finally, the litmus may be reduced and become colorless. The culture will then look milk white.Materials1. Litmus Milk Broth 2. Bunsen Burner 3. Inoculation Loop 4. wholesome slant 5. Goggles 6. Lab Coat 7.Incubator Procedure1. Get an Inoculation loop and sterilize it using the Bunsen burner. 2. Take bacteria from your nutritious slant on inoculation loop 3. Inoculate a litmus milk tube with the bacteria. 4. Incubate for 72 hours observe results every 24 hours for 3 days.